Figure 3

Immunohistochemistry on different samples. A: MB relapse, R2 immunolabelling, similar to primitive MB specimens, 40× objective. B: ETANTR, R2 immunolabelling (red). Arrows indicate three structures not seen in MB specimens. 100× objective. C to E: Ewing's sarcoma relapse. C and D: same field, 40× objective C: R1 immunolabelling in the form of fine dots; this kind of labelling was not present in MB samples. D: small autofluorescence granuli, albeit not numerous, were present in this specimen; autofluorescence was not present in primitive MB that did not undergo therapy. E: R2 immunolabelling, 20× objective. F: primary culture obtained from Ewing's sarcoma relapse; anti-R1 immunohistochemistry, 40× objective. Bar = 10 μm (B); bar = 25 μm (A, C, D, F); bar = 50 μm (E).