Characterization of the apoptotic response of human leukemia cells to organosulfur compounds

BMC Cancer

Table 1 Colony growth of leukemia cells and normal bone marrow in response to treatment with organosulfur compounds

Group	Compound Name	Structure	Dose (μM)	Selectivity
				AML	NBM
I	F2	CH₃SO₂CH₂CH₂SSCH₂CH₂SO₂CH₃	ND
	F3	CH₃SO₂CH₂CH₂SSPh	ND
	H1	CH₃OC(O)CH₂CH₂SSCH₃	ND
II	F	CH₃SO₂CH₂CH₂SSCH₃	100	++	+
	F1	CH₃SO₂CH₂CH₂SSCH₂CO₂CH₃	100	+++	+
	H	CH ₃ OC(O)CH ₂ SSCH ₃	175	++	-
	H2	CH₃OC(O)CH₂SSCH₂CH₂CH₃	50	++	+
	H5	CH ₃ OC(O)CH ₂ SS(CH ₂ ) ₆ CH ₃	200	+++	-
	H6	CH₃(CH₂)₅OC(O)CH₂SSCH₂CH₂CH₃	50	+	+
	K	CH₃SO₂CH₂SSCH₂CH₂CH₂CH₂CH₃	50	+++	++
	L	CH ₃ SO ₂ SPh	50	++	-
	N	CH ₃ SCH ₂ SSCH ₃	100	++	-
	N2	CH₃SCH₂SSCH₂CH₂CH₂SO₂CH₃	50	++	+
	O	CH ₃ OC(O)CH ₂ SSCH ₂ C(O)OCH ₃	100	++	-
	P	PhC(O)CH ₂ SSCH ₃	100	++	-
	Q	PhSSCH₂OC(O)CH₂CH₃	50	++	++
III	F4	CH₃SO₂CH₂CH₂SS(C₆H₄)OCH₃	20	++	+
	F5	CH₃SO₂CH₂CH₂SS(C₆H₄)NO₂	20	+++	+++
	F6	CH₃(C₆H₄)SO₂CH₂CH₂SSCH₃	40	+++	+++
	F7	CH₃(C₆H₄)SO₂CH₂CH₂SSPh	30	++	+
	F8	CH₃(C₆H₄)SO₂CH₂CH₂SSCH₂CH₂SO₂(C₆H₄)CH₃	20	+++	++
	H3	CH₃OC(O)CH₂SS(C₆H₄)OCH₃	ND
	H4	CH₃OC(O)CH₂SSPh	25	+	-
	H7	CH₃(CH₂)₅OC(O)CH₂S SPh	25	+	-
	H8	CH₃OC(O)CH₂SS(C₆H₄)NO₂	ND
	M	O₂N(C₆H₄)SSCH₃	50	+++	+++
	N1	CH₃SCH₂SSCH₂SCH₂SO₂CH₃	50	+++	+++

Results of compounds assayed for specificity and sensitivity to leukemic cell lines and normal bone marrow using colony growth assay. The colony growth assay results for compounds K, L, M, N, O, P and Q were repeated a minimum of three independent times with consistent results. Colony growth assay results for the remaining compounds were repeated twice. Data is summarized as the fraction of colonies formed compared to solvent controls, and indicated as >0.75 (-, no effect), 0.75 (+), 0.50 (++), and >0.25 (+++). Compounds with tumor-specific activity are shaded. Abbreviations: AML, Acute Myelogenous Leukemia; NBM, normal bone marrow; ND, not determined

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