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Figure 2 | BMC Cancer

Figure 2

From: Simplifying the detection of MUTYH mutations by high resolution melting analysis

Figure 2

Difference plot and sequence traces for MUTYH exon 13 mutations. (a) Difference plot and sequence traces for MUTYH exon 13 mutations. (a) The melting profile with sensitivity 0,45. The figure shows that mutations were clearly distinct from the wild type controls. R426C heterozygote and G396D heterozygote have identical heteroduplex melting patterns. Melt curves of each mutation (green: G396D homozygotes, pink: E410GfsX43 heterozygotes, blue: R426C heterozygotes and G396D heterozygotes, grey: double heterozygote G396D and E410GfsX43 against the wild type (red). (b) Sequencing electropherograms show a G396D heterozygous mutation, a G396D homozygous mutation and a wild type.

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