Table 2 MUTYH HRM and sequencing primer sequences
From: Simplifying the detection of MUTYH mutations by high resolution melting analysis
Exon | Primer name | Sequence | Amplicon size (base pairs) |
|---|---|---|---|
7 | 7F | 5'-GGGACTGACGGGTGATCTCT-3' | 186 bp |
7R | 5'-TTGGAGTGCAAGACTCAAGATT-3' | ||
12 | 12F | 5'-AGCCCTCTTGGCTTGAGTA-3' | 297 bp |
12R | 5'-TGCCGATTCCCTCCATTCT-3' | ||
13 | 13F | 5'-AGGGCAGTGGCATGAGTAAC-3' | 296 bp |
13R | 5'-GGGTCAAGGGGTTCAAATAG-3' |