Antibody | Parent | MRP1-tf | MRP2-tf | MRP3-tf | MRP4-tf | MRP5-tf |
---|
Negative Control | 0 | 0 | 0 | 0 | 0 | 0 |
Anti-MRP1 | 0 | 2+ | 0 | 0 | 0 | 0 |
Anti-MRP2 | 0 | 0 | 2+ | 0 | 0 | 0 |
Anti-MRP3 (M3II-9) | 0 | 0 | 0 | 2+ | 0 | 0 |
Anti-MRP3 (16A11) | 0 | 0 | 0 | 2+ | 0 | 0 |
Anti-MRP3 (1708) | 0 | 0 | 0 | 2+ | 0 | 0 |
Anti-MRP4 | 0 | 0 | 0 | 0 | 2+ | 0 |
Anti-MRP5 | 0 | 0 | 0 | 0 | 0 | 2+ |
- *Parent, untransfected lines and their single MRP-transfected, derived lines (-tf) were grown on Lab-Tek chambers for IHC analysis. Primary antibodies and irrelevant controls were used at a concentration of 12.5 μg/ml (MAbs) or 10 μg/ml (polyvalent serum 1708 or normal rabbit IgG). Columns are headed by the target cell line designation; rows are labeled to the left with the primary antibody used for specific MRP detection. 0, 2+: levels of reactivity of the antibodies with each defined MRP-expressing cell line.