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Table 3 Immunohistochemical analysis of defined MRP-expressing cell lines*

From: MRP3: a molecular target for human glioblastoma multiforme immunotherapy.

Antibody

Parent

MRP1-tf

MRP2-tf

MRP3-tf

MRP4-tf

MRP5-tf

Negative Control

0

0

0

0

0

0

Anti-MRP1

0

2+

0

0

0

0

Anti-MRP2

0

0

2+

0

0

0

Anti-MRP3 (M3II-9)

0

0

0

2+

0

0

Anti-MRP3 (16A11)

0

0

0

2+

0

0

Anti-MRP3 (1708)

0

0

0

2+

0

0

Anti-MRP4

0

0

0

0

2+

0

Anti-MRP5

0

0

0

0

0

2+

  1. *Parent, untransfected lines and their single MRP-transfected, derived lines (-tf) were grown on Lab-Tek chambers for IHC analysis. Primary antibodies and irrelevant controls were used at a concentration of 12.5 μg/ml (MAbs) or 10 μg/ml (polyvalent serum 1708 or normal rabbit IgG). Columns are headed by the target cell line designation; rows are labeled to the left with the primary antibody used for specific MRP detection. 0, 2+: levels of reactivity of the antibodies with each defined MRP-expressing cell line.