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Figure 2 | BMC Cancer

Figure 2

From: Expression of RNA interference triggers from an oncolytic herpes simplex virus results in specific silencing in tumour cells in vitro and tumours in vivo

Figure 2

Silencing in glioma cells in vitro and in vivo. (a) Rat 9L/LacZ [30] cells were infected at an MOI of 1.0 with Onc miR-neg and incubated for 48 hrs. GFP levels were assessed by fluorescent microscopy. (b) Rat 9L/LacZ cells were infected at an MOI of 1.0, 5.0 and 10.0 with Onc miR-LacZ or Onc miR-neg, and incubated for 48 hrs. B-galactosidase protein levels (units/ml) were assessed using high sensitivity β-galactosidase assay (Stratagene). P values were obtained using unpaired student t test. (c,d) Rat 9L/LacZ cells were implanted (1×107 cells) subcutaneously in the right flank of BALB/c nude mice (Harlan laboratories). Tumours were allowed to develop for 2 weeks to an average diameter of approximately 0.4-0.6 cm and then injected with 108 pfu (100 μl) of either Onc miR-LacZ or Onc miR-neg, and further incubated for 72 hrs. B-galactosidase protein levels were either assessed by standard (c) Western blot techniques or (d) high sensitivity β-galactosidase assay kit (Stratagene).

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