Figure 2From: Expression of RNA interference triggers from an oncolytic herpes simplex virus results in specific silencing in tumour cells in vitro and tumours in vivo Silencing in glioma cells in vitro and in vivo. (a) Rat 9L/LacZ [30] cells were infected at an MOI of 1.0 with Onc miR-neg and incubated for 48 hrs. GFP levels were assessed by fluorescent microscopy. (b) Rat 9L/LacZ cells were infected at an MOI of 1.0, 5.0 and 10.0 with Onc miR-LacZ or Onc miR-neg, and incubated for 48 hrs. B-galactosidase protein levels (units/ml) were assessed using high sensitivity β-galactosidase assay (Stratagene). P values were obtained using unpaired student t test. (c,d) Rat 9L/LacZ cells were implanted (1×107 cells) subcutaneously in the right flank of BALB/c nude mice (Harlan laboratories). Tumours were allowed to develop for 2 weeks to an average diameter of approximately 0.4-0.6 cm and then injected with 108 pfu (100 μl) of either Onc miR-LacZ or Onc miR-neg, and further incubated for 72 hrs. B-galactosidase protein levels were either assessed by standard (c) Western blot techniques or (d) high sensitivity β-galactosidase assay kit (Stratagene).Back to article page