Figure 5

ADAMTS1 regulates vascular function in vitro. (A) HUVECs were treated with V CM or P CM for 0.5, 1, 2, 3, 4, 6, 16 and 24 hrs and ADAMTS1 mRNA expression was determined by quantitative RT-PCR analysis. (B) HUVECs were treated with V CM, P CM or P CM incubated with IgG or ADAMTS1 antibody for immunoneutralisation. Endothelial cell proliferation was assessed as described in the materials and methods. (C) HUVECs were transfected with control siRNA or ADAMTS1 siRNA for 48hrs or left untransfected for 48 hours. Thereafter ADAMTS1 mRNA expression was investigated by quantitative RT-PCR analysis. (D) HUVECs were transfected with control siRNA or ADAMTS1 siRNA for 48hrs. Subsequently, HUVECs were treated with V CM or P CM and proliferation assays were performed as described in the materials and methods. * represents statistical significance; P < 0.05. Data are represented as mean ± SEM from at least 3 independent experiments.