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Figure 8 | BMC Cancer

Figure 8

From: Transcriptional profiling of ErbB signalling in mammary luminal epithelial cells - interplay of ErbB and IGF1 signalling through IGFBP3 regulation

Figure 8

Effect of siRNA-mediated knockdown of ErbB2 expression on IGF1-stimulated signalling. A. Control siRNA and siErbB2-transfected C3.6 cells were serum starved for 48 hrs and then stimulated with 25 ng/mL IGF1 for the indicated times. Activation of ERK1/2 and Akt was assessed by immunoblotting with phospho-specific antibodies and protein levels checked by re-probing membranes with non-phospho-specific, ErbB2, IGFBP3 and beta-actin antibodies. B. Control siRNA and siErbB2-transfected C3.6 cells were serum starved for 48 hrs and then stimulated with 25 ng/mL IGF1 for 20 min (+) or left unstimulated (-). Lysates were immunoblotted as in A. C. Control siRNA and siIGFBP3-transfected HB4a cells were serum starved for 48 hrs and then stimulated with 25 ng/mL IGF1 for 20 min (+) or left unstimulated (-). Lysates were immunoblotted as in A.

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