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Figure 4 | BMC Cancer

Figure 4

From: P53 in human melanoma fails to regulate target genes associated with apoptosis and the cell cycle and may contribute to proliferation

Figure 4

Inhibition of P53 reduces proliferation in melanoma cells. (A) Proliferation was analysed in melanocytes and WS-1 fibroblasts that had been stably transduced with P53 shRNA or control shRNA over a 72 hour period using the MTT assay. Results are represented as the mean ± SE of 3 experiments. (B) Proliferation was analysed in IgR3 and Mel-RM cells that had been stably transduced with P53 shRNA or control shRNA over a 72 hour period using the MTT assay. Results are represented as the mean ± SE of 3 experiments. (C) and (D) Proliferation was analysed by colony formation assay in IgR3 and Mel-RM cells that had been stably transduced with P53 shRNA (black bars) or control shRNA (grey bars) and compared to their parental counterparts (white bars). Representative results are shown in (C) and quantification of 3 independent experiments is shown in (D) with the number of colonies expressed as a percentage of the control shRNA transduced cell lines (mean ± SE). *p < 0.001 by students t-test.

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