Figure 4

TGFβ-induced EMT cells with CSC characteristics. (A) Phase-contrast images of treated and untreated epithelial cells after 48 hours of TGFβ stimulation (20 ×). (B) Representative images of wound healing assay of TGFβ treated and untreated epithelial cells. Data represent mean ± SEM of triplicates, *p < 0.01. (C) Tumorsphere formation assay of TGFβ treated and untreated epithelial cells. Cells were cultured in low adherent plates for two weeks (4 × magnification). (D) Relative expression of mRNA encoding EMT and TISCs genes normalized to endogenous control Gadph after TGFβ stimulation. Data represent mean ± SEM of triplicates, *p < 0.01. (E) Western blot analysis of CD44, Snail1, E-Cadherin, Nanog, and β-actin.