Method | Gene | Forward primer (5′>3′) | Fluorescent (5′3′) | Reverse primer (5′>3′) |
---|---|---|---|---|
bisulfite sequencing† | HOPX-β | TAGTITTGTTTGGAAGAGGGGCG | AACCTCCCCTAAAAACAAACTTAAC | |
Q-MSP‡ | HOPX-β | TTTGGAGAGGGTTTTAAAGCG | FAM-CGGAGATAGAAGGTCGTTTATCGGGGAGGTCG-TAMRA | AACAAACTTAACAAATCGCGAA |
Q-MSP‡ | β-actin | TGGTGATGGAGGAGGTTTAGTAAGT | FAM-ACCACCACCCAACACACAATAACAAACACA-TAMRA | AACCAATAAAACCTACTCCTCCCTTAA |
RT-PCR§ | HOPX-α and γ | CAAACCCAGGGCTTGCGCTT | GCGGAGGAGCGAAACAGAGAT | |
RT-PCR§/Q-RT-PCR | HOPX-β | GGTCCCCCTTTCGGGAGGAA | GCGGAGGAGAGAAACAGAGAT | |
RT-PCR§/Q-RT-PCR | HOPX-core | CAGAGGACCAGGTGGAAATCC | GCGGAGGAGAGAAACAGAGAT | |
RT-PCR§/Q-RT-PCR | β-actin | GCTCGTCGTCGACAACGGCTC | CAAACATGATCTGGGTCATCTTCT | |
PCR for cloning# | HOPX | CACCATGTCGGCGGAGACCGCGAGCGG | GTCTGTGACGGATCTGACACTCTG |