Skip to main content
Figure 1 | BMC Cancer

Figure 1

From: A closed-tube methylation-sensitive high resolution melting assay (MS-HRMA) for the semi-quantitative determination of CST6 promoter methylation in clinical samples

Figure 1

The primers of MS-HRM and MSP assays for CST6 promoter methylation. The MS-HRM primers are shown in red. The outer MSP primers are framed, while the inner ones are shown in purple. The region from −162 up to +188 is depicted (+1 is the Transcriptional start site). Note that this sequence is produced after bisulfite conversion of genomic DNA. All CpGs are considered to be methylated, and therefore are unaffected during the conversion process.

Back to article page

BMC Cancer

ISSN: 1471-2407

Contact us