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Figure 1 | BMC Cancer

Figure 1

From: miR-17-5p targets the p300/CBP-associated factor and modulates androgen receptor transcriptional activity in cultured prostate cancer cells

Figure 1

Expression of PCAF in cultured cell lines. A and B, expression of PCAF as assessed by Western blot and qRT-PCR, respectively. Representative Western blots are shown in A; β-actin was also blotted to ensure equal loading. The amount of PCAF mRNA is shown in B. Data in B are averages of three independent experiments. *, p < 0.05 compared to RWPE1 and PrEC cells. C and D, expression of PCAF protein in RWPE1 and LNCaP cells as assessed by immunofluorescent staining. PCAF was stained in green using a specific anti-PCAF antibody (in D). Cell nuclei were stained in blue with DAPI and overlaid images were also shown. The non-specific IgG was used as the control (as shown in C). Bar = 10 μm.

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