Table 1 Immunostaining protocol details listed by antigen
|  | CD31 | Collagen IV | αSMA | LYVE-1 |
|---|---|---|---|---|
NGS blocking incubation time | 1 h | 1 h | 1 h | 20 min |
Primary antibody dilution | 1:200 | 1:1000 | 1:1000 | 1:1000 |
Secondary antibody incubation time | 1 h | 30 min | 30 min | 30 min |
ABC reagent incubation time | 1 h | 30 min | 30 min | 30 min |