Figure 3
Inhibition of TRPC6 gene expression decreases cellular migration and invasion. (A and B) Wound healing assays were performed in SCC38, SCC40 and SCC42B cells. The rate of front migration of cell monolayers was analyzed by time-lapse video microscopy. At least 15 different fields were randomly chosen across the wound length. Values are mean of average ± s.d. from three independent experiments. (C and E) SCC42B cells treated with control (Ci) or TRPC6 siRNA (TRPC6i) were seeded in serum-free media in the upper chamber of Matrigel transwells. The lower chamber was loaded with regular media supplemented with 10% fetal bovine serum and 5% BSA. After 24 h at 37°C in 5% CO2, the top filter was scraped, and invading cells were fixed and stained. (C) Representative images captured with a 10 objective 24 h after seeding. (E) All invading cells were counted under x10 magnification. Values are mean of average ± s.d. from three independent experiments done in triplicate. (D) Inhibition of TRPC6 expression in SCC42B cells attenuates cell migration. Wound healing assays were performed in cells treated with TRPC6- (TRPC6i) or control-siRNA (Ci). Values are mean of average ± s.d. from three independent experiments.