Figure 1

Semi-quantification of immunostaining by digital image analysis. (A) The original photograph acquired from tissue sections. Black arrow indicates the positive staining cells, and white arrow indicates the negative staining stroma area. (magnification×200). (B) Marking the sections. The positive staining cytoplasm that is chosen as the color of interest and masked with red color. (C) The area of positive staining which is marked by red lines (excluded the noisy spots less than 50 pixels). The exact number of positive staining area which labeled by green spots. (D) The area of positive staining which is marked by red lines (excluded the noisy spots less than 50 pixels). The exact number of positive staining IOD which is labeled by green spots. IOD divided number of positive staining area is mean-density that represented the concentration of TFPI-2 expression.