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Figure 1 | BMC Cancer

Figure 1

From: Metallothionein 1G functions as a tumor suppressor in thyroid cancer through modulating the PI3K/Akt signaling pathway

Figure 1

MT1G inactivation mediated by epigenetic alterations in thyroid cancer. (A) Frequent down-regulation and promoter methylation of MT1G in thyroid cancer cell lines. MT1G expression was determined by semi-quantitative RT-PCR with β-actin as an internal control (upper panel). Promoter methylation of MT1G in cell lines was determined by MSP (lower panel). Mk, DNA marker; P, positive control for methylated gene. (B) MT1G expression was restored in thyroid cancer cells treated with 5-Aza-dC and SAHA alone or in combination. Real-time quantitative RT-PCR was performed to evaluate mRNA expression of MT1G, and 18S mRNA was used as a normalized control. Data are presented as mean ± SD of values from three different assays.

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BMC Cancer

ISSN: 1471-2407

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