Figure 4From: Overexpression of heat shock transcription factor 1 enhances the resistance of melanoma cells to doxorubicin and paclitaxel HSF1 silencing does not influence doxorubicin resistance in mouse melanoma B16F10 cells. A. Detection of transcripts of Hsf1 and Hsp genes in cells expressing control scrambled shRNA or HSF1-specific shRNAs (HSF1-1, HSF1-2). Where indicated, cells were subjected to heat shock (HS) for 1 h at 42°C with subsequent recovery at 37°C for 30 minutes. B. Western blot detection of HSF1 and HSPs in cells expressing control scrambled shRNA or HSF1-specific shRNAs (HSF1-1, HSF1-2). HSF1 was detected directly after HS while HSPs were detected after 6 hours recovery. C. Viability of cells treated with various concentrations of doxorubicin for 48 h. Results of the MTT assay are shown in relation to the untreated cells; mean values ± SD from three independent experiments are presented.Back to article page