Skip to main content
Figure 4 | BMC Cancer

Figure 4

From: E-cadherin gene re-expression in chronic lymphocytic leukemia cells by HDAC inhibitors

Figure 4

Chromatin Immunoprecipitation analysis (ChIP) and HDAC expression analysis. A. ChIP analysis of two CLL specimens (± HDACi). Cells were treated with MS-275 at a concentration of 1μM for 24 hours and sheared chromatin immunoprecipitated with two different antibodies, pan acetyl-H3 and pan acetyl-H4 followed by PCR amplification of the E-cadherin promoter and exon 11 regions. Real time PCR was performed to determine the percentage of input DNA (mean ± SD, data from two experiments) immunoprecipitated by the acetylated histone antibodies B. Real time PCR analysis data on the relative expression level of HDAC1-9 in three CLL specimens relative to control PBMC RNA, data also adjusted to actin.

Back to article page