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Figure 3 | BMC Cancer

Figure 3

From: Functional proteomic analysis reveals the involvement of KIAA1199 in breast cancer growth, motility and invasiveness

Figure 3

KIAA1199 Knockdown enhanced apoptosis in vitro . A) Flow cytometry analysis shows a large increase in the percentage of cells programmed for apoptosis in MDA-MB-231-ShA, MDA-MB-231-ShB, Hs578T-ShA and Hs578T-ShB cells comparing to the corresponding negative controls. B) Confirmation of the results of Flow cytometry analysis by Western blot (single experiment). Caspase-3 activation is detected in Western blots by the presence of cleavage fragments. The antibody detects both pro (full-length) and active (cleaved) protein. The increased representation of cleaved caspase-3 in KIAA1199 knockdown cells compared to the control cells is qualitatively shown in MDA-MB-231 (left panel) and Hs578T (right panel) cells.

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