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Figure 3 | BMC Cancer

Figure 3

From: Expression of inwardly rectifying potassium channels (GIRKs) and beta-adrenergic regulation of breast cancer cell lines

Figure 3

Comparison of GIRK1 mRNA expression levels by relative competitive RT-PCR in MDA-MB-453 cells treated with propranolol constantly for 6 days. Propranolol (1 μM) was added daily for six days. cDNA was amplified by PCR using GIRK1 primers and 18S primers/competimers. Lanes 1–5) untreated control; Lanes 6–10) propranolol treated cells; Lane 11) untreated control, RT reaction without MMLV; Lane 12, MDA-MB453 treated with propranolol, RT reaction without MMLV. Densitometry values were determined using the phosphoimager. Densitometry values of the bands for Girk 1 were normalized by the densitometry values of the bands for the 18S primers/competimers. Normalized values for control were 0.5494 ± 0.0285 (SD); and normalized values for propranolol treated were 0.9028 ± 0.0348 (SD), p < 0.0001. The bands were consistent with the expected sizes, 441 bp for the GIRK 1 primers and 324 bp for the 18S primers/competimers.

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