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Figure 1 | BMC Cancer

Figure 1

From: Analysis of folylpoly-γ-glutamate synthetase gene expression in human B-precursor ALL and T-lineage ALL cells

Figure 1

FPGS transcription initiation rate and start sites in CCRF-CEM and NALM6 cells. (A) Nuclear run-on assays. Intact nuclei were isolated from CCRF-CEM and NALM6 cells, and nascent RNA transcripts were labeled with [α-32P]UTP, purified, and hybridized to 1 μg of FPGS and 18S cDNAs blotted on nitrocellulose membrane. Integration densitometry values of FPGS and 18S signals were determined using the Gel-Pro program. Bars represent the mean of four independent experiments ± S.E.M. Comparison of transcription rates were achieved using a Student's t-test (*, p < 0.05). (B) 5'-RACE products of FPGS mRNA from RCH (RCH-ACV), REH, NALM6, CEM (CCRF-CEM) and K562 cells are indicated. The lanes "CTRL" and "MW" represent negative control and the 100 bp DNA ladder, respectively. See Materials and Methods for details.

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