- Research article
- Open Access
- Open Peer Review
HIV and pre-neoplastic and neoplastic lesions of the cervix in South Africa: a case-control study
© Moodley et al; licensee BioMed Central Ltd. 2006
- Received: 03 February 2006
- Accepted: 23 May 2006
- Published: 23 May 2006
Cervical cancer and infection with human immunodeficiency virus (HIV) are both major public health problems in South Africa. The aim of this study was to determine the risk of cervical pre-cancer and cancer among HIV positive women in South Africa.
Data were derived from a case-control study that examined the association between hormonal contraceptives and invasive cervical cancer. The study was conducted in the Western Cape (South Africa), from January 1998 to December 2001. There were 486 women with invasive cervical cancer, 103 control women with atypical squamous cells of undetermined significance (ASCUS), 53 with low-grade squamous intraepithelial lesions (LSIL), 50 with high-grade squamous intraepithelial lesions (HSIL) and 1159 with normal cytology. Odds ratios (OR) and 95% confidence intervals (CI) were calculated using multiple logistic regression.
The adjusted odds ratios associated with HIV infection were: 4.4 [95% CI (2.3 – 8.4) for ASCUS, 7.4 (3.5 – 15.7) for LSIL, 5.8 (2.4 – 13.6) for HSIL and 1.17 (0.75 – 1.85) for invasive cervical cancer. HIV positive women were nearly 5 times more likely to have high-risk human papillomavirus infection (HR-HPV) present compared to HIV negative women [OR 4.6 (95 % CI 2.8 – 7.5)]. Women infected with both HIV and high-risk HPV had a more than 40 fold higher risk of SIL than women infected with neither of these viruses.
HIV positive women were at an increased risk of cervical pre-cancer, but did not demonstrate an excess risk of invasive cervical cancer. An interaction between HIV and HR-HPV infection was demonstrated. Our findings underscore the importance of developing locally relevant screening and management guidelines for HIV positive women in South Africa.
- Human Immunodeficiency Virus
- Cervical Cancer
- Human Immunodeficiency Virus Infection
- Invasive Cervical Cancer
- Human Immunodeficiency Virus Status
Cervical cancer and infection with human immunodeficiency virus (HIV) are both major public health problems in South Africa. The country currently faces one of the worst HIV epidemics in the world. In the past 15 years the epidemic has grown exponentially, with antenatal HIV surveillance data showing an increase in prevalence from less than 1% in 1990 to 29.5 % in 2004 . There are currently more than 5 million people with HIV/AIDS in South Africa, with women more severely affected than men .
Cancer of the cervix is the second commonest cancer among South African women, with an age standardized incidence rate of 30 per 100 000 per year . Cervical cancer is included as one of the defining conditions of the acquired immune deficiency syndrome (AIDS) . Studies have shown that HIV positive women are at an increased risk of developing cervical squamous intraepithelial lesions (SIL) and cervical cancer [4–12]. In developed countries it is recommended that HIV positive women be screened for SILs more frequently than HIV negative women . These recommendations are not feasible in developing countries, where both HIV and cervical cancer are common, and resources limited. To develop locally feasible guidelines, data are needed on the prevalence and risk of SILs and cervical cancer among HIV positive women in South Africa. Here we report on one of the first studies that provides information on the risk of SILs among HIV positive women in the Western Cape Province in South Africa.
Data were derived from a case-control study that examined the association between hormonal contraceptives and invasive cervical cancer. The study was conducted in the Western Cape from January 1998 to December 2001. Detailed methods have been described elsewhere . There were 524 cases and 1541 controls. Refusal rates were 0.4% and 6.0 %, respectively. Cases were all Coloured and Black women with first occurrences of histologically confirmed invasive cervical cancer, identified at two tertiary gynaecology oncology clinics. The terms Coloured and Black are used to denote groups defined by race classification legislation formerly used in South Africa. In this paper the term Black refers to African people and Coloured to people of mixed racial ancestry.
Nurse interviewers enrolled controls from the local Community Health Centres and the 2 tertiary hospitals. The controls were selected to be representative of the same population from which the cases were identified. They were frequency-matched to the cases for decade of age, ethnic group and area of residence at a ratio of 3:1 controls per case. The trained nurse interviewers collected data on a wide range of variables using a structured questionnaire. Papanicolaou (Pap) smears were taken from the controls and classified according to the Bethesda system . Endocervical scrapings were taken from the controls only and were assayed for human papillomavirus (HPV) infections using the Hybrid Capture II HPV test for the detection of high-risk HPV (HR-HPV) types. The cervical cancer cases were considered positive for HR-HPV. At the time of the study serum samples were taken and stored.
The study was later extended to explore the relationship between HIV and cervical pre-cancer and cancer. Anonymous HIV testing was done on stored serum samples, using the enzyme-linked immunoabsorbant assay (ELISA). HIV data were available for 486 (93%) of the 524 cases of cervical cancer, and HIV and Pap results for 1365 (89%) of the original controls.
For the purposes of the present analysis we divided the patients into several groups. There were 486 women with invasive cervical cancer, 103 control women had atypical squamous cells of undetermined significance (ASCUS), 53 had low-grade squamous intraepithelial lesions (LSIL), 50 had high-grade squamous intraepithelial lesions (HSIL) and 1159 had normal cytology.
Written informed consent for the overall study was obtained from all study participants. Ethical approval for the overall study and, later, for the anonymous HIV testing was granted by the University of Cape Town Research Ethics Committee and the Boston University Ethics Review Board.
Socio-demographic and reproductive characteristics were examined using chi square and Kruskal-Wallis tests. The median ages of HIV positive and HIV negative women were compared using the Wilcoxan sign rank test. For the association between HIV and invasive cervical cancer we compared HIV prevalence in the cases with that in all controls. For the association between HIV and SIL, women with normal cytology were used as a reference group. Statistical analysis was carried out using unconditional multiple logistic regression (SAS version 9.1). Initial models contained multiple variables that were considered to be potential confounders (age, ethnic group, number of sexual partners, education level, urban or rural residence, marital status, presence of sexually transmitted infection, number of prior Pap smears, parity, age at coitarche, alcohol use, tobacco use, sterilization, use of oral contraceptives, and use of injectable contraceptives). A backward stepwise regression was performed. Variables were retained in the model if they demonstrated independent associations with the outcome of interest, or if their removal altered the association between HIV infections and the outcome of interest. Systematic elimination of variables resulted in odds ratio (OR) estimates adjusted for age and ethnic group.
An additional analysis, which took into account joint HIV/HR-HPV status was conducted. In this analysis women were stratified women according to whether they were HR-HPV positive or negative and HIV positive or negative. In this analysis we controlled for age and ethnicity.
Socio-demographic and reproductive characteristics
Socio-demographic and reproductive characteristics
Cases (N = 486)
Controls (N = 1 365)
< 35 years
≥ 35 years
1 146 (84.0)
1 030 (75.5)
1 105 (81.0)
< 16 years
≥ 16 years
1 008 (73.8)
Hormonal contraceptive use
1 168 (85.6)
Prior Pap smear
HIV and invasive cervical cancer
Overall 5.7% (78) of the HIV tested controls (1365) and 6.0% (29) of the tested cases (486) were HIV positive. The odds ratio for the association between HIV and invasive cervical cancer was 1.17 (95% CI 0.75 – 1.85), estimates adjusted for age and ethnicity.
Among women with cervical cancer, those that were HIV positive were 6 years younger that those that were HIV negative (median age 40 years and 46 years for HIV positive and HIV negative women respectively, p = 0.004).
Overall, the majority of women with cervical cancer presented with late stage disease (stages III and IV). Analysis of the prevalence of HIV infection according to stage of cancer showed that 8.7% (12/138) of stage Ib and II were HIV infected compared to 4.9% (17/346) of stage III and IV cancers (p = 0.17).
HIV and SIL
HIV status and risk of SIL
HIV positive N (%)
HIV negative N (%)
Adjusted OR* (95% CI)
1 120 (97.0)
4.4 (2.3 – 8.4)
7.4 (3.5 – 15.7)
5.8 (2.4 – 13.6)
Among women with pre-cancerous lesions, HIV positive women tended to be younger, but the age differences were not statistically significant for ASCUS or HSIL and only of borderline significance for LSIL (p = 0.05).
HIV, high-risk HPV and cervical lesions
HIV, HR-HPV and risk of SIL
SIL absent N
SIL present N (%)
Adjusted OR* (95% CI)
HIV – HR-HPV-
23 (2.1 %))
5 (12.5 %)
6.5 (2.3 – 33.0)
57 (29.5 %)
19.6 (11.7 – 33.0)
18 (47.4 %)
41.3 (18.8 – 90.5)
This study provides data on the risk of cervical SILs and invasive cervical cancer among HIV positive women less than 60 years old in the Western Cape Province in South Africa. The extent to which HIV increases the risk for cervical cancer is especially important in South Africa given the size of the HIV epidemic. Studies have produced inconsistent results on the association between HIV and invasive cervical cancer [10–12]. In our study HIV positive women did not have an excess risk of invasive cervical cancer. This could be due to the competing risk of mortality from other conditions associated with HIV, particularly in a setting where antiretroviral therapy was not available at the time.
Similar to other studies conducted in South Africa, the majority of women in our study, irrespective of HIV status presented with late stage disease . We found no association between HIV and the stage of cervical cancer. HIV positive women with invasive cervical cancer were 6 years younger than HIV negative women. This difference in mean age between HIV positive and negative women is difference in mean age is smaller than that reported in other South African studies [16, 17] and probably reflects the earlier stage of the HIV epidemic in the Western Cape compared to the other study sites.
Our finding that HIV infected women were at a significantly higher risk of LSIL and HSIL confirms the findings of studies from both developed and developing countries [4–9, 11]. The prevalence (50%) of cytological abnormalities among HIV positive women is higher than has been reported by any other study in Africa. Hawes et al reported a cytological abnormality prevalence of 37% among women with HIV-1 infection attending an outpatient infectious-disease clinic in Senegal , Chirenje 26 % among family planning attendants in Zimbabwe , Laga 27% in sex workers in Zaire , Maggwa 5% among family planning attendants in Kenya  and Kreiss 26% among sex workers in Kenya . The controls in the case-control study (from which our subjects were derived) were frequency matched for age to cases of invasive cervical cancer, therefore, our study consists of slightly older women than those in the above mentioned studies. This difference in age could account in part for the higher prevalence of cervical abnormality recorded in our study.
A potential limitation of our study is that cytological abnormalities were not histologically confirmed. However, it has been shown that the Pap smear sensitivity and specificity are similar among HIV negative and HIV positive women [4, 21]. Further, the cytologists were blinded to HIV status and there is no reason to suspect differential misclassification according to HIV status. Random misclassification would dilute the estimated effect of HIV. An additional limitation of our study was that immune status was not recorded. A strong association between immune status (CD4 counts and HIV viral load) and cervical abnormalities has been demonstrated in other studies [5, 8, 9].
In developed countries it is recommended that HIV positive women have two cytological assessments within the first year after HIV diagnosis and annually thereafter, with referral for colposcopy for any smear showing an ASCUS or more severe lesion . These guidelines are not feasible in resource-poor settings. The high prevalence and risk of cervical abnormalities documented in our study underscores the importance of developing screening and management guidelines for HIV positive women. As anti-retroviral therapy becomes increasingly available in the public sector in South Africa, the life expectancy of HIV positive women will increase. It is important that this benefit is not offset by an excess risk in cervical cancer. It is of critical importance that a cervical screening program, informed by local research on the natural history of cervical abnormalities, is in place for HIV positive women.
South Africa has recently introduced a national cervical screening policy that all women 30 years and older are entitled to three free Pap smears in their lifetime, at 10 year intervals . Over the next few years many women in South Africa will be screened for the first time for cervical abnormalities. According to the policy all women with LSIL should have a repeat smear in 1 year, and all women with HSIL referred for a colposcopy assessment. Based on our study findings that 1 in 4 women with LSIL, and 1 in 5 women with HSIL, were HIV positive, we recommend that every effort should be made to ensure that women who are screened and found to have SILs should also be offered HIV voluntary counselling and testing.
Our results are in agreement with others that have shown an association between HIV, high-risk HPV and cervical abnormalities [8, 23]. For the combination of HIV and HPV infection, we observed an interaction with an estimated 41-fold increase in the risk of LSIL and HSIL combined. This striking increase underscores the need to study the mechanisms underlying the interaction in the pathogenesis of cervical SIL.
In summary South African HIV positive women are at a significantly increased risk of cervical HR-HPV infection and SILs. The increased public health burden that this poses is an important and gender-specific aspect of HIV infection. Guidelines on how best to screen HIV positive women for cervical abnormalities are urgently needed in South Africa.
We would like to thank our colleagues at the hospitals in the Western Cape for their co-operation and support, Ms D Constant for assistance with statistical analysis and our fieldworkers for their contributions. The work was supported by grants from Bristol-Myers Squibb HIV/AIDS Research Institute and the National Institutes of Health, USA – Grant number R01 CA 73985-01. The funding agencies had no involvement in the research process.
- Department of Health of South Africa: National HIV and syphilis antenatal sero-prevalence survey in South Africa, 2004. 2005, PretoriaGoogle Scholar
- Mqoqi N, Kellett P, Sitas F, Musa J: Incidence of histologically diagnosed cancer in South Africa, 1998 –9. National Cancer Registry of South Africa. 2004, JohannesburgGoogle Scholar
- Centers for Disease Control and Prevention: 1993 revised classification system for HIV infection and expanded surveillance case definition for AIDS among adolescents and adults. JAMA. 1993, 269: 729-730. 10.1001/jama.269.6.729.View ArticleGoogle Scholar
- Wright TC, Ellerbrock TV, Chiasson MA, Van Devanter N, Sun XW, the New York Cervical Disease Study: Cervical intraepithelial neoplasia in women infected with human immunodeficiency virus: prevalence, risk factors and the validity of Papanicolau smears. Obstet Gynecol. 1994, 84: 591-597.PubMedGoogle Scholar
- Schuman P, Ohmit SE, Klein RS, Duerr A, Cu-Uvin S, Jamieson DJ, Anderson J, Shah KV, the Epidemiology Research Study (HERS) Group: Longitudinal Study of Cervical Squamous Intraepithelial Lesions in Human Immunodeficiency Virus (HIV)-Seropositive and At-Risk HIV-Seronegative Women. JID. 2003, 188: 128-136. 10.1086/375783.View ArticlePubMedGoogle Scholar
- Hawes SE, Critchlow CW, Niang MAF, Diouf MB, Diop A, Toure P, Kasse AA, Dembele B, Sow PS, Coll-Seck AM, Kuypers JM, Kiviat NB: Increased Risk of High-Grade Cervical Squamous Intraepithelial Lesions and Invasive Cervical Cancer among African Women with Immunodeficiency Virus Type 1 and 2 Infections. JID. 2003, 188: 555-563. 10.1086/376996.View ArticlePubMedGoogle Scholar
- Chirenje ZM, Loeb L, Mwale M, Nyamapfeni P, Kamba M, Padian N: Association of Cervical SIL and HIV-1 Infection among Zimbabwean women in an HIV/STI prevention study. Int J STD AIDS. 2002, 13 (11): 765-8. 10.1258/095646202320753727.View ArticlePubMedGoogle Scholar
- Massad LS, Ahdieh L, Benning L, Minkoff H, Greenblatt RM, Watts H, Miotti P, Anastos K, Moxley M, Muderspach LI, Melnick S: Evolution of cervical abnormalities among women with HIV-1: Evidence from surveillance cytology in the Women's Interagency HIV Study. J Acquir Immun Defic Syndr. 2001, 27: 432-442.View ArticleGoogle Scholar
- Delmas MC, Larsen C, Van Benthem B, Hamers FF, Bergeron C, Poveda JD, Anzen B, van den Hoek A, Meier F, Pena JM, Savonius H, Sperandeo D, Suligoi B, Vernazza P, Brunet JB, De Vincenzi I, for the European Study Group on Natural History of HIV Infection in Women: Cervical squamous intraepithelial lesions in HIV-infected women: prevalence, incidence and regression. AIDS. 2000, 14: 1775-1784. 10.1097/00002030-200008180-00013.View ArticlePubMedGoogle Scholar
- Sitas F, Pacella-Norman R, Carrara H, Patel M, Ruff P, Sur R, Jentsch U, Hale M, Rowji P, Saffer D, Connor M, Bull D, Newton R, Beral V: The spectrum of HIV-1 related cancers in South Africa. Int J Cancer. 2000, 88: 489-492. 10.1002/1097-0215(20001101)88:3<489::AID-IJC25>3.0.CO;2-Q.View ArticlePubMedGoogle Scholar
- La Ruche G, You B, Mensah-Ado I, Bergeron C, Montcho C, Ramon R, Toure-Coulibaly K, Welffens-Ekra C, Dabis F, Orth G: Human Papillomavirus and Human Immunodeficiency Virus Infections: Relation with cervical dysplasia-neoplasia in African Women. Int J Cancer. 1998, 76: 480-486. 10.1002/(SICI)1097-0215(19980518)76:4<480::AID-IJC6>3.0.CO;2-N.View ArticlePubMedGoogle Scholar
- Gichangi PB, Bwayo J, Estambale B, De Vuyst H, Ojwang S, Rogo K, Abwao H, Temmerman M: Impact of HIV infection on invasive cervical cancer in Kenyan women. AIDS. 2003, 17: 1963-1968. 10.1097/00002030-200309050-00015.View ArticlePubMedGoogle Scholar
- Centers for Disease Control: 1997 USPHS/IDSA Guidelines for the prevention of opportunistic infections in persons infected with human immunodeficiency virus. MMWR. 1997, 46: 1-26.Google Scholar
- Shapiro S, Rosenberg L, Hoffman M, Kelly J, Cooper D, Carrara H, Denny LE, du Toit G, Allan BR, Stander IA, Williamson A-L: Risk of invasive cancer of the cervix in relation to use of injectable progestogen contraceptives and combined estrogen/progestogen contraceptives (South Africa). Cancer Causes and Control. 2003, 14: 485-495. 10.1023/A:1024910808307.View ArticlePubMedGoogle Scholar
- Solomon D, Davey D, Kurman R, Moriarty A, O'Connor D, Prey M, Raab S, Sherman M, Wilbur D, Wright TW, Young N, for the Forum Group Members and the Bethesda 2001 Workshop: The 2001 Bethesda System; Terminology for Reporting Results of Cervical Cytology. JAMA. 2002, 287: 2114-2119. 10.1001/jama.287.16.2114.View ArticlePubMedGoogle Scholar
- Moodley M, Moodley J, Kleinschmidt I: Invasive cervical cancer and human immunodeficiency virus (HIV) infection: a South African perspective. Int J Gynecol Cancer. 2001, 11: 194-197. 10.1046/j.1525-1438.2001.01022.x.View ArticlePubMedGoogle Scholar
- Lomalisa P, Smith T, Guidozzi F: Human Immunodefiency Virus Infection and Invasive Cervical Cancer in south Africa. Gynecologic Oncology. 2000, 77: 460-463. 10.1006/gyno.2000.5775.View ArticlePubMedGoogle Scholar
- Laga M, Icenogle JP, Marsella R, Manoka AT, Nzila N, Ryder RW, Vermund SH, Heyward WL, Nelson A, Reeves WC: Genital Papillomavirus infection and cervical dysplasia – Opportunistic Complications of HIV Infection. Int J Cancer. 1992, 50: 45-48.View ArticlePubMedGoogle Scholar
- Maggwa BN, Hunter DJ, Mbugua S, Tukei P, Mati JK: The relationship between HIV infection and cervical intraepithelial neoplasia among women attending two family planning clinics in Nairobi, Kenya. AIDS. 1993, 7 (5): 733-738.View ArticlePubMedGoogle Scholar
- Kreiss JK, Kiviat NB, Plummer FA, Roberts PL, Waiyaki P, Ngugi E, Holmes KK: Human Immunodeficiency Virus, human papalliomavirus, and cervical intraepithelial neoplasia in Nairobi prostitutes. Sex Transm Dis. 1992, 19: 54-59.View ArticlePubMedGoogle Scholar
- Branca M, Rossi E, Alderisio M, Migliore G, Morosini PL, Vecchione A, Sopracordevole F, Mudu P, Leoncini L, Syrjanen K: Performance of cytology and colposcopy in diagnosis of cervical intraepithelial neoplasia (CIN) in HIV-positive and HIV-negative women. Cytopathology. 2001, 12: 84-93. 10.1046/j.1365-2303.2001.00299.x.View ArticlePubMedGoogle Scholar
- National Department of Health (NDOH): National guidelines for cervical cancer screening programme. Pretoria, South Africa. 2000Google Scholar
- Ahdieh L, Klein RS, Burk R, Cu-Uvin S, Schuman P, Duerr A, Safaeian M, Astemborski J, Daniel R, Shah K: Prevalence, Incidence, and Type-specific Persistence of Human Papillomavirus in Human Immunodeficiency Virus (HIV)-Positive and HIV-Negative Women. JID. 2001, 184: 682-90. 10.1086/323081.View ArticlePubMedGoogle Scholar
- The pre-publication history for this paper can be accessed here:http://0-www.biomedcentral.com.brum.beds.ac.uk/1471-2407/6/135/prepub
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