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Figure 4 | BMC Cancer

Figure 4

From: Bcl-XL is qualitatively different from and ten times more effective than Bcl-2 when expressed in a breast cancer cell line

Figure 4

Prevention of apoptosis in Rat-1 cells by Bcl-2 and Bcl-XL and by ER localized mutants. A) Cell lysates containing 20 μg of protein from vector control (neo) or transfected Rat-1 cells expressing wild type proteins or ER localized mutants (Bcl2-cb5, BclX-cb5) as indicated above the lanes were immunoblotted for PARP and actin. Cells were exposed to etoposide or low serum. Untreated vector transfected cells (-). The migration position of the 89 kDa caspase cleaved PARP fragment is indicated as ΔPARP. B) Rat-1 cells transfected with the control plasmid (Neo -) or stably expressing the indicated protein and treated with TNFα and cycloheximide were stained with Hoescht 33342 or Annexin V coupled to Alexa Fluor594 (as indicated above the panels) and visualized by epifluorescence microscopy. A few normal nuclei are indicated by open arrowheads while examples of condensed nuclei are indicated by solid arrowheads. The width of each image is ~660 μm. C) Quantification of the results from experiments similar to those shown in B). Each determination represents 450–600 cells analyzed converted to % positive by dividing by the total number of Hoescht stained cells, independent of whether chromatin condensation was observed.

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