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Figure 5 | BMC Cancer

Figure 5

From: Targeting of mutant hogg1in mammalian mitochondria and nucleus: effect on cellular survival upon oxidative stress

Figure 5

mtDNA integrity after oxidative damage exposure. MtDNA integrity of indicated mitochondrial regions in HeLa cells transfected with empty vector, MTS-hOGG1 and MTS-Mutant hogg1 and treated with (A) 400 μM of H2O2 for 2 h; (B) 28 μM of Adriamycin for 2 h and (C) 50 μM of 4-NQO for 1 h were analyzed by using quantitative real-time PCR amplification. The extent of decrease in mtDNA integrity was analyzed by calculating the mtDNA/nuclear DNA ratio, and normalizing to the untreated control set at 100%. The error bars represent standard deviation of each experiment done twice in triplicates. An asterisk indicates a significant difference (0.004 ≥ p ≥ 0.0001), when compared to the vector using Student's t test.

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