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Figure 5 | BMC Cancer

Figure 5

From: Down-regulation of SFRP1 as a putative tumor suppressor gene can contribute to human hepatocellular carcinoma

Figure 5

The correlation between the expression of SFRP1 gene and DNA methylation status of SFRP1 promoter. (A) DNA methylation status of SFRP1 promoter was assessed in some HCC cell lines with or without the expression of endogenetic SFRP1, as well as fetal and adult normal livers, through MSP assay with specific primers. The peripheral blood lymphocyte (PBL) DNA treated with SssI Methylase (New England Biolabs, Beverly, MA) was used as a positive control for methylation (IVD), and water was used as a negative control (H2O). (B) Bel7402, QGY7701 and MHCC-H cells without the expression of endogenous SFRP1 were treated with 5-aza-2'-deoxycytidine (DAC) and trichostatin A (TSA) alone or in combination. The expression of SFRP1 was then evaluated by RT-PCR. Untreated HCC cells were employed as control (lane 1). (C) The DNA methylation status of SFRP1 promoter in these cells treated by DAC and TSA were further evaluated by MSP assay and the same primers, where bisulfite-treated genomic DNA from these specimens was used as template. M, methylated; U, unmethylated.

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