Figure 6
From: FRA-1 protein overexpression is a feature of hyperplastic and neoplastic breast disorders
Immunohistochemical and RT-PCR analysis of the fra-1 gene expression in fine needle aspiration biopsy samples (FNAB). The cytological specimens investigated by immunohistochemistry were also analyzed by RT-PCR. The mRNAs were extracted from FNABs of breast tissues and amplified by RT-PCR using fra-1 specific primers as specified under "Methods". The cDNAs were co-amplified with gapdh gene as an internal control. A) Immunostaining in a FNAB deriving from a normal breast tissue (100 ×), and from a carcinoma samples (100 ×) (B). C) A carcinoma FNAB was incubated without the primary antibody (100 ×) as control of the specificity of the antibodies. D) Fra-1 expression was analyzed by RT-PCR. The sources of RNAs were: lane 1: normal breast; lane 2, breast dysplasia; lanes 3, 4 and 5: breast carcinomas; lane -, water control. Molecular markers are indicated.