Figure 3
From: Conjugated linoleic acid induces apoptosis through estrogen receptor alpha in human breast tissue
CLA induces apoptosis in ERα positive human breast cancer cell line. (A) ERα protein expression was examined by western blot of whole cell lysate isolated from MCF-7, MDA-MB-231 (parental), and MDA-MB-231-ERα (ERα transfected MDA-MB-231). Equal amounts of isolated protein from cell extracts of all three cell lines were subjected to immunoblot with anti-ERα antibodies. β-actin was used as loading control. Effects of CLA on apoptosis of MCF-7, MDA-MB-231, and MDA-MB-231-ERα were determined by Hoechst staining (B) and Apo-ONE® homogeneous caspase-3/7 assay (C). Bars represent mean ± SD, n = 3. *p < 0.05 stands for control versus 40 μM t10, c12-CLA.