Figure 9

Relative effect of vapor and particulate ('tar') phases of cigarette smoke on eIF2α phosphorylation. NHBE cells were exposed to air (mock treatment M), whole cigarette smoke (W), vapor phase (V) or particulate 'tar' phase (T) from 2R4F cigarettes, after which the cells were placed in fresh medium and returned to the incubator for the time periods specified. Vapor phase was created by passing whole smoke through a Cambridge pad filter to trap the particulate matter. Particulate 'tar' phase was achieved by passing the whole smoke through 450 mg activated carbon to remove vapor phase components. Duration of whole smoke and vapor phase exposures was 20 minutes. Duration of particulate 'tar' phase exposures was 25 minutes in order to deliver an amount of particulate matter equivalent to that of the whole smoke exposure as some particulate matter is lost upon transit through the activated carbon. In all exposures the 35 cc puffs were diluted in 250 cc air. Western blots of whole cell lysates were probed with antibodies to phosphorylated eIF2α, eIF2α, and GAPDH.