Figure 3

IGF-I -induced caspases activation is neutralised by anti-IGF-IR antibodyIGF-I-induced activation of caspases 3/7 (a, b), 8 (c, d) and 9 (e, f) in HT-29 cells and caspase 3/7 in SW620 cells (g, h) is neutralised by anti-IGF type 1 receptor antibody (IGF-IR ab). Cells were pre-incubated with (+) or without (-) IGF-IR ab (400 ng/ml) for 30 minutes and then treated with (+) or without (-) IGF-I (50 ng/ml) for 48 hours. The cellular caspase 3/7, 8 and 9 activities were analysed with Caspase-Glo assay kit (Promega, Madison USA). To check whether any IGF-IR antibody neutralization of IGF-I action is a specific effect a general mouse IgG was included (i, j). For mouse IgG control experiment cells were pre-incubated with (+) or without (-) mouse IgG (400 ng/ml) for 30 minutes. The caspase 3/7 activity was analysed with the same method as IGF-IR ab. Significance value: * P <0.05; ** P <0.01. SCM-serum containing media; SFM-serum free media.