Figure 4

Exogenous IGF-I dose nod change cell death and viability in HT-29, SW620 and HCT116 cells. HT-29 (a), SW620 (b) and HCT116 (c) cells were treated with different concentrations of 5-Fluorouracil (5-FU) (0, 37.5, 75, 150 and 300 μM) for 48 hours. The cell death index was determined by a previously reported method [13]. HT-29 (d), SW620 (e) and HCT116 (f) cells were treated with IGF-I (100 ng/ml) for 48 hours and cell death index was determined with the same method. HT-29 (g), SW620 (h) and HCT116 (i) cells were treated with IGF-I (100 ng/ml) for 48 hours and cell viability was determined by a previously reported cell proliferation assay [20].