Skip to main content
Figure 5 | BMC Cancer

Figure 5

From: IGF-I activates caspases 3/7, 8 and 9 but does not induce cell death in colorectal cancer cells

Figure 5

Inhibition of IGF-I-induced caspases activation does not affect colorectal cancer cell proliferation. The IGF-I-induced caspase activation is inhibited by incubation with Z-VAD-FMK (a general caspase inhibitor) for 48 hours. The caspase 3/7 activity was measured with Caspase-Glo assay kit (Promega, Madison USA). Cell proliferation was measured with Alamar blue (Serotec Ltd, Oxford, UK). It was shown that Z-VAD-FMK indeed was able to inhibit caspase 3 activation in HT-29 (a), SW620 (c) and HCT116 cells (e). But the inhibition has no effect on cell proliferation (b, d and f).

Back to article page