- Research article
- Open Access
- Open Peer Review
The mRNA expression of SETD2 in human breast cancer: correlation with clinico-pathological parameters
© Al Sarakbi et al; licensee BioMed Central Ltd. 2009
- Received: 30 March 2009
- Accepted: 21 August 2009
- Published: 21 August 2009
SET domain containing protein 2 (SETD2) is a histone methyltransferase that is involved in transcriptional elongation.
There is evidence that SETD2 interacts with p53 and selectively regulates its downstream genes. Therefore, it could be implicated in the process of carcinogenesis. Furthermore, this gene is located on the short arm of chromosome 3p and we previously demonstrated that the 3p21.31 region of chromosome 3 was associated with permanent growth arrest of breast cancer cells. This region includes closely related genes namely: MYL3, CCDC12, KIF9, KLHL18 and SETD2. Based on the biological function of these genes, SETD2 is the most likely gene to play a tumour suppressor role and explain our previous findings.
Our objective was to determine, using quantitative PCR, whether the mRNA expression levels of SETD2 were consistent with a tumour suppressive function in breast cancer. This is the first study in the literature to examine the direct relationship between SETD2 and breast cancer.
A total of 153 samples were analysed.
The levels of transcription of SETD2 were determined using quantitative PCR and normalized against (CK19).
Transcript levels within breast cancer specimens were compared to normal background tissues and analyzed against conventional pathological parameters and clinical outcome over a 10 year follow-up period.
The levels of SETD2 mRNA were significantly lower in malignant samples (p = 0.0345) and decreased with increasing tumour stage.
SETD2 expression levels were significantly lower in samples from patients who developed metastasis, local recurrence, or died of breast cancer when compared to those who were disease free for > 10 years (p = 0.041).
This study demonstrates a compelling trend for SETD2 transcription levels to be lower in cancerous tissues and in patients who developed progressive disease. These findings are consistent with a possible tumour suppressor function of this gene in breast cancer.
- Breast Cancer
- Human Breast Cancer
- Tumour Suppressive Function
- Tumour Suppressor Role
- Breast Cancer Specimen
Histone-lysine N-methyltransferase (SETD2) is a protein that methylates 'Lys-36' of histone H3. H3 'Lys-36' methylation represents a specific tag for epigenetic transcriptional activation. 
We previously demonstrated that the 3p21.31 region of chromosome 3 was associated with permanent growth arrest of breast cancer cells. This region includes closely related genes namely: MYL3, CCDC12, KIF9, KLHL18 and SETD2. Based on the biological function of these genes, SETD2 is the most likely gene to play a tumour suppressor role and explain our previous findings .
SETD2 plays a role in chromatin structure modulation during transcriptional elongation via its interaction with hyperphosphorylated POLR2A. It also may act as a transcription activator that binds to promoters. 
SETD2 was first implicated in Huntington's disease (HD). Huntington's disease is a neurodegenerative disorder characterized by loss of striatal neurons, is caused by an expansion of a polyglutamine tract in the HD protein Huntingtin.  This gene encodes a protein belonging to a class of huntingtin interacting proteins characterized by WW motifs. [5, 6] This protein is a histone methyltransferase that is specific for lysine-36 of histone H3, and methylation of this residue is associated with active chromatin. This protein also contains a novel transcriptional activation domain and has been found associated with hyperphosphorylated RNA polymerase II.
It has been established that Histone modifications, which include acetylation, phosphorylation, methylation and ubiquitination, play key roles in gene regulation.  These modifications create both agonistic and antagonistic signals that correlate with the transcriptional activity of a gene, through engaging some protein complexes or through changing the structure of chromatin to allow access for RNA polymerase to initiate transcription.  These Histone modifications could act as marking system that is responsible for establishing and maintaining programs of gene expression during cellular differentiation.
Previous research has shown that SETD2 could interact with p53 and selectively regulate the transcription factor activity of p53. The interaction was dependent of C-terminal region of SETD2, which contains the SET and WW domains and the N-terminal trans-activation domain of p53 . This evidence demonstrated that SETD2 down-regulated hdm29 (transcription subset gene) expression by targeting its P2 promoter and then enhanced p53 protein stability. These findings suggest that SETD2 could selectively regulate the transcription of subset genes via cooperation with the transcription factor p53.  So, hypothetically, increased SETD2 activity could enhance the action of tumour suppressor gene P53 and therefore have tumour suppressive function in human breast cancer.
Furthermore, SETD2 is located on the short arm of chromosome 3 and this region has been associated with permanent growth arrest of the tumour cells. 
The aim of our study was to determine, using quantitative PCR, whether the mRNA expression levels of SETD2 were consistent with a tumour suppressive function in human breast cancer. This is the first study in the literature to examine the direct relationship between SETD2 and breast cancer.
Patients & Samples
Clinical data showing number of patients in each category
Alive With metastasis
With local recurrence
Died of breast cancer
Died of unrelated disease
ER receptor status
RNA extraction kits and reverse transcription kits were obtained from Sigma-Aldrich Ltd (Poole, Dorset, England, UK). PCR primers were designed using Beacon Designer (Palo Alto, CA) and synthesized by Sigma-Aldrich. Custom made hot-start Master-mix for quantitative PCR was obtained from Abgene (Surrey, England, UK). [9, 10]
Tissue Processing, RNA Extraction and cDNA Synthesis
Frozen sections of tissue were cut at a thickness of 5–10 mm and kept for routine histological analysis. Additional 15–20 sections were mixed and homogenized using a hand-held homogenizer in ice-cold RNA extraction solution. The concentration of RNA was determined using UV spectrophotometry. Reverse transcription was carried out using a reverse transcription kit with an anchored olig (dT) primer supplied by Abgene, using 1 mg of total RNA in a 96-well plate. The quality of cDNA was verified using b-actin primers (Appendix 1).
The level of SETD2 transcripts from the above prepared DNA were determined using real-time quantitative PCR based on the Amplifluor technology, modified from a method reported previously.  PCR primers were designed using Beacon Designer software, but to the reverse primer an additional sequence, known as the Z sequence (5'-ACTGAACCTGACCGTACA-3') which is complementary to the universal Z probe (Intergen, Inc., Oxford, UK) was added. The product expands one intron. The primers used for each SETD2 are detailed in Appendix 1. The reaction was carried out using the following: Hotstart Q-master mix (Abgene), 10 pmol of specific forward primer, 1 pmol reverse primer which has the Z sequence, 10 pmol of FAM-tagged probe (Intergen, Inc.), and cDNA from 50 ng of RNA. The reaction was carried out using the IcyclerIQ (Bio-Rad Ltd, Hemel Heamstead, England, UK), which is equipped with an optic unit that allows real-time detection of 96 reactions, under the following conditions: 94°C for 12 min and 50 cycles of 94°C for 15 sec, 55°C for 40 sec, and 72°C for 20 sec. The levels of the transcript were generated from a standard that was simultaneously amplified with the samples. Levels of SETD2 expression were then normalized against CK19 expression, already measured in these specimens, to correct for varying amounts of epithelial tissue between samples. CK19 transcripts were quantified as previously reported,  using primers detailed in Appendix 1. With every PCR run, we included a negative control without a template and a known cDNA reference sample as a positive control.
The Mann-Whitney U test was used for statistical analysis. SETD2 transcript levels within breast cancer specimens were compared to normal background tissues and analyzed against conventional pathological parameters and clinical outcome over a 10 year follow-up period. In each case the true copy number was used for statistical analysis and hence samples were not classified as positive or negative. Statistical analysis was carried out using Minitab (14.1) using a custom written macro (Stat 2005.mtw).
Levels of SETD2 mRNA expression (After CK19 normalisation) in each group of patients
Mean ± SD
SETD2 all tissue
85547 ± 498012
414434 ± 1106629
20950 ± 37626
8755 ± 26203
16517 ± 55868
318 ± 645
2592 ± 9686
11942 ± 35360
11457 ± 44706
8551 ± 29766
8578 ± 24043
174 ± 414
679 ± 1081
12239 ± 43466
Alive with Metastasis
1983 ± 5142
3513 ± 5683
Died of Br Ca
698 ± 1741
ER receptor status
4742 ± 18706
14278 ± 46482
1698 ± 4441
13353 ± 43908
Firstly, we examined the correlation between expression levels and tumour stage. We found a marked decrease in SETD2 levels correlating with higher stages of tumour. This was statistically significant when comparing TNM1 to TNM3 (p = 0.038), TNM1 to TNM4 (p = 0.05), and TNM2 toTNM3 (p = 0.05). We then examined the correlation with tumour grade. There was a noticeable trend with lower levels correlating with increasing tumour grade; however, this did not reach statistical significance (p = 0.16 and 0.18 when comparing grade 1 to grade 2 and 3 respectively).
We then examined the correlation with the Nottingham Prognostic Index (NPI) and also noted a distinct decrease in levels with NPI scores. This, however, was only statistically significant when comparing NPI1 to NPI 3 (p = 0.026).
p values for SETD2 mRNA expression levels in relation to survival and disease progression
Disease free>10 years
Alive with Metastasis
p = 0.062
p = 0.13
Died of Br Ca
p = 0.0026
All progressive disease
p = 0.041
Data analysis also showed a correlation with ER status (Estrogen receptors in Ductal carcinoma patients). Levels of mRNA were significantly lower in ERb negative samples when compared to positive (p = 0.025). Although data analysis showed no statistically significant correlation between SETD2 expression levels and ERą (p = 0.16).
This is the first study in the literature to examine the direct relationship between SETD2 mRNA expression and breast cancer. Our findings demonstrate that this gene may have a tumour suppressor function and its mRNA expression could play a role as a prognostic indicator in human breast cancer.
SETD2 (SET domain containing protein 2) is a histone H3K36 trimethyltransferase protein that associates with hyperphosphorylated RNA polymerase II and involves in transcriptional elongation.
It has been established that regulation of chromatin structure modulates DNA replication and transcription. One major mechanism that regulates the structure and function of chromatin is the covalent modification of histones, [12, 13] and this is linked to SETD2 function. Several studies suggest that SETD2 and modified histones are linked to transcriptional activation and repression, DNA repair, and cell cycle regulation. [14, 15]
Previous research showed that SETD2 could interact with p53 and selectively regulate the transcription factor activity of p53.  Abnormalities of the p53 tumour suppressor gene are among the most frequent molecular events in human neoplasia.  Tumour suppressor p53 acts as transcriptional activator, controlling the expression of a variety of genes important in cell cycle regulation and apoptosis.  It is composed of four identical subunits, binds to a specific site on the DNA, and interacts with transcription interaction factors, leading to the initiation of transcription by RNA polymerase II. Furthermore, SETD2 could be co-immunoprecipitated with p53 under both exogenous and endogenous conditions. Therefore, SETD2 over-expression augmented expression of the majority of p53 target genes and SETD2 suppression led to their down-regulation. 
Such studies support and explain our finding of a compelling trend for SETD2 mRNA levels to be lower in cancerous tissues and in patients who developed progressive disease (distal metastasis, local recurrence, or died of breast cancer). These observations are consistent with a possible tumour suppressor function of this gene in breast cancer. Furthermore its location on chromosome 3p is also consistent with such possible role. We also demonstrated a statistically significant correlation with ERb status which is a favourable prognostic indicator in human breast cancer.
The strength of our report lies in the use of robust RT-PCR methodology to analyze SETD2 mRNA expression in a cohort of breast cancer patients with a long-term follow up.
We are the first group to investigate SETD2 expression in human breast cancer and identify a possible TSG function. However there are several inherent limitations to our study related to the relatively small sample size and lack of data regarding protein expression of the gene studied.
Further research is required to confirm the role of SETD2 gene in the pathogenesis of breast cancer including immunohistochemistry studies, in vitro experiments and the preparation of animal models with suppressed SETD2 gene.
If our observations are confirmed by other studies, SETD2 could prove a valuable prognostic marker and its artificial expression could represent a novel therapeutic strategy.
Primers for SETD2F1
Primers for SETDZr1
Primers for SETD2F2
Primers for SETD2Zr2
Primers for SETD2F3
Primers for SETD2Zr3
Primers for SETD2F4
Primers for SETDZr4
- Sun XJ, Wei J, Wu XY, Hu M, Wang L, Wang HH, et al: Identification and characterization of a novel human histone H3 lysine 36-specific methyltransferase. J Biol Chem. 2005, 280 (42): 35261-71. 10.1074/jbc.M504012200.View ArticlePubMedGoogle Scholar
- Cuthbert AP, Bond J, Trott DA, Gill S, Broni J, Marriott A, et al: Telomerase repressor sequences on chromosome 3 and induction of permanent growth arrest in human breast cancer cells. J Natl Cancer Inst. 1999, 91 (1): 37-45. 10.1093/jnci/91.1.37.View ArticlePubMedGoogle Scholar
- Li M, Phatnani HP, Guan Z, Sage H, Greenleaf AL, Zhou P: Solution structure of the Set2-Rpb1 interacting domain of human Set2 and its interaction with the hyperphosphorylated C-terminal domain of Rpb1. Proc Natl Acad Sci USA. 2005, 102 (49): 17636-41. 10.1073/pnas.0506350102.View ArticlePubMedPubMed CentralGoogle Scholar
- Rega S, Stiewe T, Chang DI, Pollmeier B, Esche H, Bardenheuer W, et al: Identification of the full-length huntingtin- interacting protein p231HBP/HYPB as a DNA-binding factor. Mol Cell Neurosci. 2001, 18 (1): 68-79. 10.1006/mcne.2001.1004.View ArticlePubMedGoogle Scholar
- Passani LA, Bedford MT, Faber PW, McGinnis KM, Sharp AH, Gusella JF, et al: Huntingtin's WW domain partners in Huntington's disease post-mortem brain fulfill genetic criteria for direct involvement in Huntington's disease pathogenesis. Hum Mol Genet. 2000, 9 (14): 2175-82. 10.1093/hmg/9.14.2175.View ArticlePubMedGoogle Scholar
- Faber PW, Barnes GT, Srinidhi J, Chen J, Gusella JF, MacDonald ME: Huntingtin interacts with a family of WW domain proteins. Hum Mol Genet. 1998, 7 (9): 1463-74. 10.1093/hmg/7.9.1463.View ArticlePubMedGoogle Scholar
- Edmunds JW, Mahadevan LC, Clayton AL: Dynamic histone H3 methylation during gene induction: HYPB/Setd2 mediates all H3K36 trimethylation. EMBO J. 2008, 27 (2): 406-20. 10.1038/sj.emboj.7601967.View ArticlePubMedGoogle Scholar
- Xie P, Tian C, An L, Nie J, Lu K, Xing G, et al: Histone methyltransferase protein SETD2 interacts with p53 and selectively regulates its downstream genes. Cell Signal. 2008, 20 (9): 1671-8. 10.1016/j.cellsig.2008.05.012.View ArticlePubMedGoogle Scholar
- Jiang WG, Douglas-Jones A, Mansel RE: Level of expression of PPAR-gamma and its co-activator (PPAR-GCA) in human breast cancer. Int J Cancer. 2003, 106: 752-757. 10.1002/ijc.11302.View ArticlePubMedGoogle Scholar
- Jiang WG, Watkins G, Lane J, et al: Prognostic value of Rho familty and and rho-GDIs in breast cancer. Clin Cancer Res. 2003, 9: 6432-6440.PubMedGoogle Scholar
- Jiang WG, Watkins G, Fodstad O, Douglas-Jones A, Mokbel K, Mansel RE: Differential expression of the CCN family members Cyr61 from CTGF and Nov in human breast cancer. Endocrine Related Cancers. 2004, 11: 781-791. 10.1677/erc.1.00825.View ArticleGoogle Scholar
- Spencer VA, Davie JR: Role of covalent modifications of histones in regulating gene expression. Gene. 1999, 240 (1): 1-12. 10.1016/S0378-1119(99)00405-9.View ArticlePubMedGoogle Scholar
- Strahl BD, Allis CD: The language of covalent histone modifications. Nature. 2000, 403 (6765): 41-5. 10.1038/47412.View ArticlePubMedGoogle Scholar
- Turner BM: Histone acetylation and an epigenetic code. Bioessays. 2000, 22 (9): 836-45. 10.1002/1521-1878(200009)22:9<836::AID-BIES9>3.0.CO;2-X.View ArticlePubMedGoogle Scholar
- Wolffe AP: Transcriptional regulation in the context of chromatin structure. Essays Biochem. 2001, 37: 45-57.View ArticlePubMedGoogle Scholar
- Prives C, Hall PA: The p53 pathway. J Pathol. 1999, 187 (1): 112-26. 10.1002/(SICI)1096-9896(199901)187:1<112::AID-PATH250>3.0.CO;2-3.View ArticlePubMedGoogle Scholar
- Goodsell DS: The molecular perspective: p53 tumor suppressor. Oncologist. 1999, 4 (2): 138-9.PubMedGoogle Scholar
- The pre-publication history for this paper can be accessed here:http://0-www.biomedcentral.com.brum.beds.ac.uk/1471-2407/9/290/prepub
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