Table 1 Forward and reverse primers for the genes analyzed
Gene | Forward primer | Reverse primer |
|---|---|---|
HIF-1α | 5'CTGCTGTCTTACTGGTCCTT3' | 5'GTCGCTTCTCCA ATTCTTAC3' |
COX-2 | 5'CCATTCAGTTCCCACCATCT3' | 5'TCACTGCTGTTGGGTCTCTG3' |
MMP7 | 5'AGATGTGGAGTGCCAGATGT3' | 5'TAGACTGCTACCATCCGTCC3' |
MMP9 | 5'GGCGCTCATGTACCCTATGT3' | 5'CCTGTGTACACCCACACCTG3' |
VEGF | 5'ATGAACTTTCTGCTCTCTGG3' | 5'TCATCTCTCCTATGTGCTGGC3' |
PDGFRA | 5'GGGGAAACGATTGTGGTCACC3' | 5'CCCGCACCTCTACAACAAAAT3' |
MYC | 5'AAAGGCCCCCAAGGTAGTTA3' | 5'TTTCCGCAACAAGTCCTCTT3' |
HPRT | 5'CCTGGCGTCGTGATTAGTG3' | 5'CAGAGGGCTACAATGTGATGG3' |
TBP | 5'ACCACTCCACTGTATCCCTCC3' | 5'CTGTTCTTCACTCTTGGCTCCT3' |