Figure 1

MIF targeting by antisense transfection results in reduced MIF protein and altered morphology of glioma cells. Human LN18 glioma cells transfected with an antisense-RNA MIF construct and corresponding antisense-expressing clones (as1 + as2) were selected (A). These cells showed a markedly reduced MIF protein expression (B) and altered cell morphology in culture compared to wildtype (wt) and empty vector transfected control cells (c1) (C). MIF expression ratios have been calculated from MIF expression relative to 18S (A) or Actin (B) in three independent experiments.