Skip to main content
Figure 5 | BMC Cancer

Figure 5

From: Oridonin stabilizes retinoic acid receptor alpha through ROS-activated NF-κB signaling

Figure 5

Oridonin-induced RARα stability requires the activation and nuclear translocation of p65. (A) NB4/GFP and NB4/GFP-MAD cells were treated with 10 μM oridonin for 12 h. The intracellular localization of p65 was analyzed using anti-p65 antibodies (red) with DAPI staining (blue) for nuclei. Scale bars represent 20 μm. (B, C) NB4/GFP and NB4/GFP-MAD cells were treated with 10 μM oridonin for 12 h (B) or treated with 5 μM H2O2 for the times indicated (C), and the cell lysates were western blotted for the indicated proteins. (D) NB4 cells were infected with pSIREN-RetroQ-derived retroviruses carrying shRNA for p65 or scrambled shRNA as a control, and the cell lysates were western blotted for the indicated proteins. (E, F) NB4-NC and NB4-sh-p65 cells were treated with 10 μM oridonin for 12 h (E) or with 5 μM H2O2 for the times indicated (F), and the cell lysates were western blotted for proteins as indicated. All experiments were repeated three times and gave consistent results.

Back to article page