Direct confirmation of quiescence of CD34+CD38- leukemia stem cell populations using single cell culture, their molecular signature and clinicopathological implications

Table 1 Relative down-regulation of genes involved in proliferative activity in ASCs, as determined by PCR array and real-time PCR

		Genes involved in proliferative activity
		BMPR1A	CREBBP	RBL2	FZD5	FGFR1	Notch 3	IL6ST	GLI1
Fold-changes in gene expression (ASCs/non-ASCs)^*		-12.8075	-11.6383	-11.3575	-12.5726	-14.0221	-12.309	-12.6345	-12.9973
Relative mRNA expression^†
	ASC 1	106.2	109.8	110.3	109.5	107.5	111.7	111.0	112.8
	ASC 2	101.4	106.9	111.2	111.9	104.7	107.0	106.1	109.1
	ASC 3	117.5	121.4	120.7	121.8	119.6	123.5	120.2	123.1
	ASC 4	98.9	103.9	102.8	106.6	101.9	104.0	103.3	107.2
	ASC 5	102.4	101.5	105.3	103.1	100.8	105.1	102.4	104.3
	ASC 6	125.7	128.9	110.9	131.7	125.7	124.7	124.5	128.9
	ASC 7	104.1	107.6	110.4	111.4	108.9	112.1	108.3	107.7
	Non ASC 1	125.8	125.1	128.7	128.3	125.2	129.6	128.4	131.5
	Non ASC 2	93.4	96.7	99.2	100.4	96.6	98.9	98.1	96.9
	Non ASC 3	125.6	134.2	127.9	132.0	130.4	131.6	128.2	133.0
	Non ASC 4	132.7	139.0	135.6	138.7	135.4	140.4	130.5	141.2
	Non ASC 5	106.0	111.4	108.7	112.6	109.2	110.8	115.0	113.8
	Non ASC 6	123.3	131.3	143.5	129.2	127.7	130.8	129.0	131.8
	Non ASC 7	122.1	127.5	126.6	129.1	124.1	121.9	126.2	129.4
	Average relative mRNA expression of ASCs	108.0	111.4	110.2	113.7	109.8	112.6	110.8	113.3
	Average relative mRNA expression of non ASCs	113.8	118.1	117.8	119.7	116.2	118.5	116.9	119.8

Abbreviations: BMPR1A Bone morphogenetic protein receptor, type IA, CREBBP CREB binding protein, RBL2 Retinoblastoma-like 2, FZD5 Frizzled family receptor 5, FGFR1 Fibroblast growth factor receptor 1, IL6ST Interleukin 6 signal transducer, GLI1 GLI family zinc finger 1.
^*Fold-changes of down-regulated gene expression in ASCs relative to non-ASCs obtained from a representative AML patient.
^†Relative mRNA expression was calculated as follows: 100 × threshold cycles of target/β-actin.

ISSN: 1471-2407