Figure 1

Platinum-based chemotherapeutic agents affect MDM2 and VEGF pre-mRNA splicing. A-D: MCF7 and Ishikawa cells were treated with the indicated concentrations of cisplatin and harvested after 24 hours. E: MCF7 cells treated with cisplatin (50 μM) were harvested at the indicated times. F: The indicated cells were treated with cisplatin (50 μM) for 24 hours. G: MCF7 cells were treated with oxaliplatin at 50 μM and harvested after 24 hours. Analysis of the splicing of MDM2 (A,B,E-G) or of VEGF-A (C,D) transcripts was performed by end-point RT-PCR and acrylamide gel electrophoresis as detailed in Methods. Illustrated gels are representative of three independent experiments. FL: Full Length; ALT1: Splice variant of MDM2.