Figure 5

SRSF4 is involved in cisplatin-induced splicing. A-B: MCF7 cells were transfected with siRNA targeting SRSF4 or/and SRSF6. Cells were harvested three days post-transfection and SRSF4 and SRSF6 mRNA levels were measured by end-point RT-PCR to control the efficiency of the siRNA. C-F: the histograms and errors bars represent mean and SD, respectively, illustrating the inter-experiment differences in the percentage of exon inclusion (n = 5 to 7). However, the statistics were made on the fold change measured in each independent experiment. MCF7 cells transfected with control siRNA (siSCR) or siRNA targeting SRSF4, SRSF6 or both were treated with cisplatin. The splicing of MDM2 (C), HNRNPDL-E6 (**p = 0.012; ***p = 0.0013) (D), HNRPDL-E8 (**p = 0.0163) (E) and AMZ2 (***p = 0.0002, **p = 0.001) (F) was evaluated by end-point RT-PCR.