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Figure 2 | BMC Cancer

Figure 2

From: Discovery and anti-cancer evaluation of two novel non-ATP-competitive FGFR1 inhibitors in non-small-cell lung cancer

Figure 2

Compounds Ad23 and Af23 inhibited intracellular FGFR1/FRS2 phosphorylation. FGFR1 over-expression 293 cells (A) or H460 cells (B and C) were pretreated with compounds at indicated concentrations or vehicle (0.1% DMSO), respectively. Then, cells were stimulated with bFGF (30 ng/mL) for 10 min, and the phosphorylation levels of FGFR1 (A and B) and FRS2 (C) in cell lysates was measured by western blot analysis. The figures were representative of 3 separate experiments. The column figures show the normalized optical density as a percentage of total protein control. Bars represent the mean ± SEM of 3 independent experiments. Statistical significance relative to bFGF alone group was expressed, *P <0.05, **P < 0.01, ***P < 0.001.

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