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Fig. 2 | BMC Cancer

Fig. 2

From: Enhanced efficacy of photodynamic therapy by inhibiting ABCG2 in colon cancers

Fig. 2

Differences of PDT effect between SW480 and HT29 cells depending on ABCG2 expression level. a, SW480 and HT29 cells were irradiated with a PDT laser (4 J/cm2) after a 16 h pretreatment of PPa at indicated concentrations. The MTT assay was performed in triplicate 24 h following the irradiation. b, Cells were incubated with 100 nM PPa for 16 h and then lysed with methanol, followed by application of PMT-based singlet oxygen monitoring system. c, Cells were incubated with 200 nM PPa for 16 h and then subjected to immunocytochemical staining for anti-abcg2 (Green), PPa (Red), and DAPI (blue). Data are means ± SEM from three independent experiments (*P < 0.05, **P < 0.01, #P < 0.0001)

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