Fig. 3
From: Enhanced efficacy of photodynamic therapy by inhibiting ABCG2 in colon cancers
Effect of Ko-143 on PPa treated colon cancer cell in PDT. a, Cells were irradiated by 670 nm light (4 J/cm2) after 16 h incubation with indicated concentration of PPa in the presence or absence of 1 μM Ko-143. At 24 h after PDT, the MTT assay was performed. b, Cells were incubated with 100 nM PPa for 16 h in the presence or absence of 1uM Ko-143 and then lysed with methanol, followed by application of PMT-based singlet oxygen monitoring system. c, Cells were incubated with 200 nM PPa for 16 h in the presence or absence of 1uM Ko-143 and then subjected to immunocytochemical staining for anti-abcg2 (Green), PPa (Red), and DAPI (blue). Data are means ± SEM from three independent experiments (*P < 0.05, **P < 0.01, #P < 0.0001)