Fig. 5
From: Enhanced efficacy of photodynamic therapy by inhibiting ABCG2 in colon cancers
Differences of singlet oxygen production rate & cell death between SW480 and SW480/ABCG2 cells. a, SW480, SW480/ABCG2, and HT29 cells were incubated with 100 nM PPa for 16 h and then lysed with methanol, followed by application of PMT-based singlet oxygen monitoring system. b, Cells were harvested 4 h after photosensitization and stained with Annexin V/PI for FACS analysis. c, Cells were incubated with 100 nM PPa for 16 h followed by irradiation. Cell lysates were subjected to immunoblot with indicated antibodies. Data are means ± SEM from three independent experiments (*P < 0.05, **P < 0.01, #P < 0.0001)