Fig. 7From: A potential small-molecule synthetic antilymphangiogenic agent norcantharidin inhibits tumor growth and lymphangiogenesis of human colonic adenocarcinomas through blocking VEGF-A,-C,-D/VEGFR-2,-3 “multi-points priming” mechanisms in vitro and in vivoNCTD inhibits migration and invasion of HT-29 cells, HDLECs and the co-culture system in vitro (inverted optic microscope, magnification × 200). a Migration assay by Transwell migration chambers: total number of migrating cells of the co-culture system was more than that of HT-29 cells or HDLECs (all *P < 0.01) in control group. After treatment with NCTD, mF4-31C1 or NCTD+ mF4-31C1, total number of migrating cells in these groups was decreased significantly as compared to control group (all #P = 0.000); of them, the number of migrating cells in NCTD+ mF4-31C1group was the least (§P = 0.01). b Invasion assay by Matrigel invasion experiment: total number of invading cells through the filter coated Matrigel of the co-culture system was also more than that of HT-29 cells or HDLECs (all*P < 0.01) in control group; the number of invading cells in NCTD, mF4-31C1 or NCTD + mF4-31C1 group was decreased significantly as compared to control group (all #P = 0.000), with the least invading cells in NCTD + mF4-31C1group (§P = 0.01)Back to article page