Fig. 8
Study of M1 (a) and M2 (b) polarization markers mRNA expression in etoposide-incubated macrophages. THP-1 macrophages were differentiated (M0) and polarized in M1 and M2 macrophages by respectively 24 h with IFN-γ (20 ng/ml) + LPS (10 pg/ml) (M1) and 72 h with IL-4 and IL-13 (20 ng/ml each- M2)). Once polarized, they were incubated in CO2-independent medium (+3,75 g D-glucose/l) during 16 h before incubation with or without 50 μM etoposide (+/− e) during 24 h. After the incubation, mRNA expression of M1 and M2 macrophage markers was studied by RT-qPCR and normalized by RPS9 expression. Results are expressed as means ± 1 S.D. (n = 3). Statistical analysis was carried out with the two-way ANOVA test followed by a Holm-Sidak post-test NS: not significantly different. *: significantly different from the corresponding control with p < 0.05; ## or ###: significantly different respectively with p < 0.01 or 0.001. Statistical analyses were performed on log-transformed data for all the genes