Fig. 1

Bajoran Purple (BJP) chromogen was used to mark CD147 expression in prostate samples. BJP was separated from the hematoxylin (HT) counterstain using inForm software (a-f) and was then quantified in the plasma membrane (g). No significant differences were observed between benign prostatic tissue (BPT; n = 46 patients) and benign prostatic hyperplasia (BPH; n = 23) samples (p = 0.15) or high-grade intraepithelial neoplasia (HGPIN; n = 24; p = 0.63). Significant decreases in expression were observed in localized prostate cancer (PCa local; n = 42; p = 0.02), aggressive prostate cancer (PCa aggr.; n = 31; p = 0.004), and metastases (Mets; n = 20; p = 0.001) compared to BPT. E-cadherin was quantified for validation of membrane segmentation (h), and significant decreases in expression were found in all PCa samples (p < 0.0001) compared to BPT but not in BPH or HGPIN (p > 0.05) *p < 0.05