Fig. 1
Clinical relevance of FATS expression in breast cancer. a Quantitative analysis of FATS mRNA (GenBank accession number: NM_001004298) in breast cancer. Tumor samples and paired (>5 cm away) normal breast tissue samples (n = 38) from the Tianjin cohort were subjected to RNA extraction and subsequent RT-PCR analysis. The average CT value for FATS gene in each sample was obtained from three independent experiments, and normalized by that of GAPDH gene to obtain ΔCT. The quantity of FATS mRNA in each sample was calculated as 2−ΔCT. b Immunohistochemistry of FATS in paired human breast tumor samples and normal breast tissue samples (n = 30). A representative picture is shown with the same magnification (40X). N, normal; T, Tumor. c The average level of FATS mRNA from three independent experiments in breast tumor samples (n = 156) from the Tissue Bank Facility of TMUCIH was profiled according to pathological stages, in comparison with that in normal breast tissues. N: normal (n = 38); T-I: TNM stage I (n = 40); T-II: TNM stage II (n = 97); T-III: TNM stage III (n = 19). *, P < 0.05; **, P < 0.01