Fig. 5From: Adenosine A2b receptor promotes progression of human oral cancerHypoxic induction of ADORA2B. a OSCC-derived cell lines are exposed to hypoxia (1 % oxygen ) and probed for ADORA2B expression by qRT-PCR. Expression of ADORA2B mRNA is induced significantly (p < 0.05, Mann–Whitney U test) by hypoxia in OSCC-derived cell lines (HSC-3 and -4). b Immunoblotting analysis shows that the ADORA2B protein levels in hypoxia also have increased markedly compared with that in normoxia. c Immunocytofluorescence labeling of ADORA2B (red). Cellular nuclei are labeled with DAPI (blue). Immunocytofluorescence shows that the ADORA2B expression levels are higher in hypoxia than in normoxia in OSCC-derived cell lines (HSC-3 and -4)Back to article page