Fig. 4

Transcriptional and secretory profiles of pro-OC factors in Everolimus-treated BC cells. RNA levels of M-CSF, TNFα, IL-β, IL-6, MIP-1α and MMP-13 were variably suppressed in both MDA-MB-231 and MCF-7 cells after treatment with Everolimus. a RT-PCR showed a significant reduction of M-CSF, TNFα, and MMP-13 RNAs in MDA-MB-231, with a concurrent diminution of IL-6. IL-1β, IL-6, MIP-1α and MMP13 were also significantly inhibited in MCF-7 cells as compared to normalized values from control untreated BC cells. Data are mean values from three experiments. b ELISA measurement of major NFkB-dependent osteoclastogenic factors, namely M-CSF, IL-6, IL-1β, TNF-α and MIP-1α, released by cultured MDA-MB-231 and MCF-7 cells before or after sub-lethal doses of Everolimus. In most instances, Everolimus suppressed the secretion of these factors (mean values from three experiments), showing a similar pattern to that of RNA inhibition