Fig. 6

Model of MIR494 cellular responses in 769-P cells. MIR494 induces increase in cleaved PARP and LC3B in 769-P cells. This response was Ago2-dependent and was reversed upon addition of anti-MIR494. MIR494 expression led to reduction in total cholesterol levels, mitochondrial changes, LC3B punctae changes, and a significant increase in lipid droplets. The increase in lipid droplets observed in MIR494 expressing cells was dependent on LC3B. ATG7 knockdown led to an increase in smaller sized lipid droplets which was not altered by MIR494 expression